Video

Bone marrow provides a rich platform for investigating immunologic hubs in the context of relapsed acute myeloid leukemia (AML) hematopoietic stem cell transplant (HSCT). An informative setting to interrogate the basis of effective immune responses and mechanisms of relapse and remission in donor lymphocyte infusion (DLI) following HSCT. However, conventional processing of clinical bone marrow samples involves formalin fixation, paraffin embedding (FFPE), and decalcification, which degrade RNA and limit opportunities for spatial transcriptomic profiling. Singular Genomics’ high-throughput in situ multiomic G4X Spatial Sequencer enables simultaneous profiling of gene transcription, protein expression, and fluorescent H&E in degraded FFPE samples.

In this webinar, Catherine J. Wu, professor of medicine and chief in the Division of Stem Cell Transplantation and Cellular Therapies at the Dana-Farber Cancer Institute, will describe her team’s application of the G4X platform to FFPE samples from clinical bone marrow biopsy specimens from patients with relapsed AML after HSCT who were treated with DLI to elucidate the spatial relationships of immune cellular networks in the marrow microenvironment. Further evaluation of these spatial interactions may lead to novel targets for therapeutically optimizing adoptive cellular immunotherapy.

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