Delivering Fast Whole Genome Sequencing Results with Improved Efficiency
Whole genome sequencing (WGS) is the process of analyzing the complete genetic makeup of an organism. WGS helps us better understand the genetic variants within intronic and exonic regions between organisms. The G4 Sequencing Platform addresses the current limitations of WGS by eliminating batching requirements, generating faster results, and seamlessly integrating into existing next-generation sequencing workflows.
High Quality Whole Genome Sequencing
The rapid run time, high read accuracy, scalable sequencing capacity, and independent handling of samples in separate flow cell lanes–in combination with the plug-and-play compatibility make G4 well-suited for whole genome experiments.
Key capabilities:
- Novel Rapid sequencing-by-synthesis (SBS) chemistry and state-of-the-art optics deliver 30x coverage of four human genomes in under 24 hours.
- Highly accurate whole genome data comparable to leading platforms with single-pass accuracy of ~99.9% and uniform coverage of the high-confidence regions.
G4 Specifications for Whole Genome Sequencing
| F2 Flow Cell | F3 Flow Cell | |
|---|---|---|
| Paired Reads Delivered / Flow Cell | 200M | 400M |
| Paired Reads Delivered / Run | 800M | 1.6B |
| # Samples / Flow Cell1 | 0.5 | 1 |
| # Samples / Run1 | 2 | 4 |
| Price / M Paired Read | $4 | $2.5 |
| Run Time | <24 hours | <24 hours |
| Quality | >85% bases ≥ Q30 | >85% bases ≥ Q30 |
The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.
1Assumes 2×150 read configuration
Performance Characterization of the G4 for Human Whole Genome Sequencing
The WGS Technical Report highlights the capabilities of the G4 to deliver results faster, more flexible whole genome sequencing results with highly accuracy base calling.
G4 Plug-and-Play Capability
We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.
Talk to an expert about Whole Genome Sequencing on the G4 today
Speak to a member of the Singular team to learn more about the available kits and capabilities for whole genome sequencing on the G4.
