G4X Platform and Ecosystem
Transforming Spatial Multiomics with Precision, Scalability and Flexibility
Tissue Transfer
Novel and Scalable Tissue Handling Methods
- Controlled tissue handling - Minimizing the impact on valuable FFPE tissue while maximizing imaging area usage.
- Reduction in tissue waste - Eliminates the need to score blocks or use tumor micro-arrays (TMAs).
- Efficient storage and transfer of tissues- Users can store tissue on hydrogel pads after sectioning or on slides after transfer.
- User-friendly, high-volume operation- Enables simple transfer of a full run of 128 samples in as little as 3 hours.
Chemistry and workflow
Innovative Chemistry, Optimized for FFPE
- Efficient Capture in FFPE Samples - High affinity, short probes enable effective RNA capture from fragmented archived clinical sample types.
- High Sensitivity for RNA and Protein Detection- Amplification-based detection of padlock probes on target RNA regions or protein.
- Proprietary Methods for Direct Sequencing - Purpose-built technology to enable gap-fill sequencing of variable RNA sequences directly in cells, in tissues (coming soon).
- Streamlined Wet Lab Workflow - Simplified 3-day workflow enabled by our purposefully-designed workflow suite.
System Operations
Flexible, High-Throughput System Operations
- Established Instrument- The G4X has a field-matured base platform with years of real-world use and customer insights as a sequencer.
- High-Speed Imaging System- Operating at over 2 billion pixels per second, G4X imaging occurs in a single day, enabling multiple runs per week.
- Flexible-High Throughput Run Capacity - 40 cm2 of tissue imaging, divided across 4 flow cells allows users to adapt the run size to their project needs.
- Independent Lanes - Each flow cell has 2 or 4 fluidically independent lanes, allowing users to run up to 16 panels per run.
Analysis
Powerful Single-Cell Analysis Suite
- Integrated Single-Cell Analysis: The G4X performs base calling, transcript identification, clustering and cell segmentation for robust single-cell results and run QC.
- Multiomic Results Viewer: A flexible web-based platform allows for layering fH&E images, protein images, and transcript localization at subcellular resolution.
- Plug-and-Play with 3rd-Party Platforms- Standard spatial file outputs empower users to leverage their preferred open-source or commercial secondary analysis tools for deeper insights
Panels
Modular, Application-Specific Panels
- Well-Curated and Performance-Optimized - Expert-selected gene sets to ensure each panel is precisely curated to characterize tissue-immune interaction and accurate cell typing.
- Modular Design for Versatility - The flexible architecture allows researchers to combine our refined core immune panel with disease-specific modules tailored to their project needs.
- Customizable for Targeted Insights - Easily add up to 25 genes to a panel, create a new 100-gene module, or create fully customized 350-gene panels to meet specific research goals.
Specifications
G4X Spatial Sequencing Platform
Component
Details
Technologies
In Situ Multiomics
Beyond in situ multiomics, G4X is capable of fast, flexible next-generation sequencing from 4-color rapid SBS chemistry.
Sequencing ?
Modalities
350-Plex RNA
Full multiomic capability will be open for early access in Q2 with broader availability the second half of the year.
15-Plex Protein?
fH&E
Direct-Seq is a novel approach for in situ sequencing of up to 100 bases of variable RNA sequence in target regions directly in tissue samples. (Coming Soon)
Direct-Seq ?
Tissue Types
Human FFPE
Coming soon
Other Tissue Types?
Off-the-Shelf Panels
Lung Cancer
Breast Cancer
Colon Cancer
Kidney Cancer
Immune Core
Customization
350-Gene New Panel
100-Gene Mix-in
25-Gene Add-on
Proteomics will be available for early access Q2, with broader availability the second half of the year.
2 Protein Add On?
Workflow
Novel Tissue Transfer
3-Day Workflow
Precision Tooling
Run Throughput
1 to 4 Flow Cells
2 to 16 Lanes
4 to 128 Samples
4 to 40 cm2
Analysis
Multimodal Viewer
3rd-Party Compatible Outputs
Clustering and cell segmentation are done onboard, resulting in single-cell sample metrics post-run. Cell segmentation is currently based on nuclear expansion. Membrane-based segmentation is in development and coming soon.
Single Cell Output ?
Note: G4X is currently in development. Specifications, configurations, and capabilities are subject to change. Please contact us for the latest updates on availability and performance.
Spatial Reagents
Flow Cell
Configuration
Throughput
Run Time1
X2s
2 Lanes
10x10 mm ROIs
4 Samples
4 cm2
29 Hours
X2
2 Lanes
10x10 mm ROIs
10 Samples
10 cm2
29 Hours
X4
4 Lanes
4.5x4.5 mm ROIs
4 Lanes
6.5 cm2
29 Hours
1Spatial runtime does not include analysis time since spatial analysis occurs after run completion. Depending on setup, analysis can be offloaded so the system can be initiated on consecutive days for near-continuous operation. If analysis is not offloaded, run time depends on number of flow cells and configurations being run, but should not exceed 3 days for fully-loaded runs.
Note: G4X is currently in development. Specifications, configurations, and capabilities are subject to change. Please contact us for the latest updates on availability and performance.
Note: G4X is currently in development. Specifications, configurations, and capabilities are subject to change. Please contact us for the latest updates on availability and performance.
Sequencing Reagents
Flow Cell
Configuration1
Throughput2
Run Time3
Quality4
F3
4 Lanes
100 Cycles
300 Cycles
400M Reads
24 Hours
85% ≥ Q30
F4
4 Lanes
100 Cycles
300 Cycles
600M Reads
24 Hours
80% ≥ Q30
1Reagents include 50 additional cycles above what is represented to account for index sequencing needs.
2Indicates typical reads passing filter. Throughput may vary depending on application and read length.
3Sequencing run time includes analysis time, and is measured from run start through clustering, sequencing, and instrument wash for non-indexed reads. Run time will vary depending on the number of flow cells and the high-performance computing configuration.
4Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on Salmonella and PhiX control libraries.
Note: G4X is currently in development. Specifications, configurations, and capabilities are subject to change. Please contact us for the latest updates on availability and performance.
2Indicates typical reads passing filter. Throughput may vary depending on application and read length.
3Sequencing run time includes analysis time, and is measured from run start through clustering, sequencing, and instrument wash for non-indexed reads. Run time will vary depending on the number of flow cells and the high-performance computing configuration.
4Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on Salmonella and PhiX control libraries.
Note: G4X is currently in development. Specifications, configurations, and capabilities are subject to change. Please contact us for the latest updates on availability and performance.
Get Started
Technology Access Services
Generate high-dimensional G4X data on your samples now through our Spatial Services Lab.
G4X Platform
G4X will be available in June. First-year instrument allocation is limited so reserve your system soon.
SIngle cells on breast cancer sample from G4X run and multimodal (RNA + Protein) unsupervised clustering
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