Sequencing on G4X
Beyond spatial, G4X is also the industry's most powerful benchtop sequencer. Providing fast, flexible NGS results across sample types.
Features
Fast, Flexible Sequencing
Daily Runs
Innovative high-speed imaging, rapid fluidics and novel chemistry work in concert to reduce run times from days to hours (under 24 hour runs across applications).
Flexible Run Sizes
Run 1, 2, 3 or 4 flow cells at a time to efficiently match run output with project requirements.
User Friendly Operation
Simple experiment management with 4 fluidically independent lanes per flow cell and tolerant to low complexity libraries and variable loading conditions.
Accurate Results
Proprietary 4-color Rapid SBS chemistry with novel nucleotides, specifically-engineered enzymes, and molecular biology methods to deliver accurate paired-read sequencing (85% bases ≥ Q30).
Workflow
Plug and Play into Existing Lab Ecosystems
Compatible with a Wide Range of Library Prep Methods
Supports diverse workflows, including leading commercial and custom library prep kits, enabling broad application flexibility across research and clinical settings.
Simple and Intuitive Run Initiation
User-friendly software interface enables streamlined setup with minimal steps, allowing for fast onboarding and consistent execution across users and sites.
Effortless Bioinformatics Integration
Standard FASTQ output ensures plug-and-play compatibility with existing pipelines, accelerating downstream analysis and minimizing data handling complexity.
Application Performance
Accurate Results Across Key Applictions
Sequencing on the G4X is compatible with a wide range of sample preparation workflows, through seamless integration into bioinformatic pipelines, the G4 is a plug-and-play solution.
Whole Genome
High accuracy, broad variant detection
Whole Genome Sequencing
Accurate whole-genome sequencing with high coverage, base quality, and variant concordance.
Targeted Somatic
Sensitive and precise variant detection
Targeted Sequencing
Rapid somatic variant detection with high concordance, sensitivity, and variant calling performance.
Single-Cell Seq
Sensitive single-cell gene expression
Single Cell Sequencing
Sensitive and consistent single-cell RNA-Seq with strong gene detection.
Bulk RNA-Seq
Consistent performance across expression studies
Bulk RNA-Seq
High performance for RNA-Seq: reproducibility, coverage uniformity, transcript detection.
Metagenomics
Reliable detection across complex microbial samples
Metagenomics
High-quality microbial whole-genome sequencing with strong coverage and read depth
Whole Exome
High coverage and uniformity for exome panels
Whole Exome Sequencing
Accurate whole-exome sequencing with high concordance, coverage depth, and uniformity.
Specifications
Sequencing on G4X
Flow Cell
Config.1
# REads2
Run Time3
Quality4
F3
100 Cycles
300 Cycles (2x150)
100M / Lane
400M / FC
1.6B / Run
12-24 Hours
85% ≥ Q30
1Reagents include 50 additional cycles above what is represented to account for index sequencing needs.
2Indicates typical paired reads passing filter. Throughput may vary depending on application and read length.
3Sequencing run time includes analysis time, and is measured from run start through clustering, sequencing, and instrument wash for non-indexed reads. Run time will vary depending on the number of flow cells and the high-performance computing configuration.
4Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on Salmonella and PhiX control libraries.
Note: G4X is currently in development. Specifications, configurations, and capabilities are subject to change. Please contact us for the latest updates on availability and performance.
2Indicates typical paired reads passing filter. Throughput may vary depending on application and read length.
3Sequencing run time includes analysis time, and is measured from run start through clustering, sequencing, and instrument wash for non-indexed reads. Run time will vary depending on the number of flow cells and the high-performance computing configuration.
4Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on Salmonella and PhiX control libraries.
Note: G4X is currently in development. Specifications, configurations, and capabilities are subject to change. Please contact us for the latest updates on availability and performance.
Learn More
REsources
Sequencing Support Hub
Learn more about about sequencing on the G4X with detailed infomation around library preparation, kit configurations, instrument operations and analysis.
Library Prep
Explore compatibility of G4X Sequencing with our library prep partners
Demo Data
Download raw G4X sequencing data across applications
Speak to an Expert
Schedule a technical seminar and get your questions answered.
Get Started
G4X Platform Adoption
Meet with our team to discuss becoming an early G4X site.
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