G4X™ Spatial Research Challenge

Apply for one of two in situ multiomic research award tracks on G4X: a funded 1,000-sample cohort study or a 3D reconstruction study using serial FFPE sections.

Both tracks include 16-plex protein, up to 1,000-plex RNA, and fH&E, with all sample processing run as a service by Singular Genomics

Get Started

3D niches from multiomic reconstruction of 10 serial renal cell carcinoma sections on single G4X flowcell (6.2 M cells).

Intro

One Challenge. Two Ways to Push Spatial Further.

The G4X Spatial Research Challenge is designed to support ambitious studies that take advantage of multimodal spatial analysis on FFPE tissue.

For researchers pursuing cohort-scale discovery, the flagship award supports a fully funded 1,000-sample study. For researchers focused on tissue architecture and three-dimensional biology, the second award track supports 3D reconstruction from serial FFPE sections.

Together, these two tracks are intended to support a broader range of high-impact studies, from large translational cohorts to spatially rich 3D tissue investigations.

Download

TRACK 1

1,000 Sample Cohort Study

A single winning proposal will receive a funded 1,000-sample multimodal spatial study run by Singular Genomics on G4X. Runner ups will be considered for additional awards.

Included in every sample

up to 1,000-plex RNA*
16-plex protein
fH&E

Designed for

cancer research
translational biomarker studies
therapy response and resistance
disease heterogeneity
large retrospective cohorts
AI-ready spatial dataset generation

Best for
Studies where cohort size, statistical power, and multimodal tissue insight are central to the scientific question.

Download

G4X Spatial Multiomic Results on Head and Neck Cancer FFPE Samples

Multiomic Single-Cell Results from Head and Neck Cancer Sample Cohort on G4X
Courtesy of Dr. Shanye Yin, Assistant Professor in the Department of Pathology at Albert Einstein College of Medicine

TRACK 2

3D Spatial Study

A single winning proposal will receive a funded 3D in situ multiomic study run by Singular on G4X.

Supported Formats (or)

8, 40 µm FFPE reconstructions (4.5x4.5mm)
4, 80 µm FFPE reconstructions (4.5x4.5mm)
2, 160 µm FFPE reconstructions* (4.5x4.5mm)
2, 50 µm FFPE reconstructions (10x10mm)

Included in every sample

up to 1,000-plex RNA*
16-plex protein
fH&E

Best for
Studies where tissue architecture, cell-cell relationships, and three-dimensional structure are central to the biological question.

Download

Pearson correlation of per-probe expression levels with positive probes (green) and negative controls between serial sections of kidney cancer FFPE sample profiled on two separate flow cells.